Protein Science

Description of research

Protein Science

The section deals broadly with proteins as the cell’s and organs’ "work horses", trying to understand the biological processes and systems from a molecular understanding of the proteins’ properties. We analyse the proteins’ physical and chemical structures and their functions under physiologically relevant conditions. Specific areas include:

Research areas and projects:

  • expression, purification and functional optimisation of enzymes (rational protein engineering and directed evolution, design of proteins)
  • folding and pathological aggregation of proteins
  • functional amyloid in bacteria: identification, purification and description of biological regulatory mechanisms
  • folding and stability of membrane proteins
  • protein interaction with membranes and surfactants
  • global proteomic analysis, including relation to various corneal disorders
  • biomarker studies in pig models of human diseases, including metabolic diseases, neurological diseases and inflammatory conditions.
  • system biological description of host-pathogen interaction at the proteome-genome and metagenome levels.
  • development of methods for absolute protein quantification.
  • structural protein studies and protein-protein interaction studies
  • transcriptome-analysis-facilitated proteomic analysis studies of species with unknown genomes
  • the extracellular matrix

Protein structure technologies:

  • structural protein studies and protein-protein interaction studies
  • spectroscopy (fluorescence, circular dichroism, FTIR, light scattering)
  • calorimetry (DSC / ITC)
  • plate reader technology
  • size separation technology (field flow fractionation)
  • surface binding (quartz crystal micro balance)
  • phospholipid vesicle technology
  • AFM
  • heterologous expression
  • chromatography
  • peptide and protein libraries

Proteomic technologies

  • mass spectrometry (MS)-based identification (LC-MS/MS and MALDI-TOF-MS)
  • quantitative MS verification of biomarkers.
  • discovery-based "shot-gun" proteomics (Q-TOF instrument).
  • hydrogen / deuterium exchange MS (Q-TOF instrument)
  • targeted proteomics (quantitative analysis of triple-Q instrument)
  • absolute quantification of proteins by SRM (selected Reaction Monitoring) and QconCAT technology.

Section coordinator

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Group leaders

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Junior group leader

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