1) Gene promoters that are far away from other genes typically produce transcripts on both strands. The PROMoter uPstream Transcript (PROMPT) is short and rapidly degraded due to special DNA sequence patterns around the PROMPT and which are not present at the gene start site. Thus, the gene product is typically a stable mRNA. 2) If two gene start sites share a common promoter, no PROMPTs are produced. Both genes start sites produce stable RNAs. 3) If two gene promoters are closely positioned, PROMPTs are produced, but are stabilized because the DNA signals necessary for their degradation cannot form because the gene promoters are too close. Instead, the PROMPTs grow longer and are stable, which in effect creates longer mRNA variants from the two gene start sites. 4) If gene promoters are sufficiently separated, their DNA patterns do not influence their neighboring PROMPTs, which remain short and unstable, much like in the first case above.

15.08.2016 | Forskning

Generation of complex gene architectures in the human genome

Intense investigations during the past 10-15 years have revealed that the human genome is transcribed in a manner that is much more complicated than previously appreciated. A collaboration between researchers from Aarhus and Copenhagen now reveals some underlying principles leading to such promiscuous genome activity.