Aarhus University Seal

AU EV Network (tentative)

The Network

The network is a local initiative started at Aarhus University to bring together researchers and students working in the highly interdiscplinary field of extracellular vesicles (EVs). The aim is to boost our research at large by joining forces across different departments/centres through collaborations and student education.

Network Meetings

To be updated

Student Meetings

To be updated

Contact Organizers

Overview of Research Infrastructure

EV Isolation and Purification

Ultracentrifugation

  • Beckman Coulter Optima L-100K, Type 60 Ti rotor (iNANO, MBG)

Specialized size-exclusion chromatography (SEC)

  • Izon qEV SEC (AUH) – through collaboration with Kim Ryun Drasbek
  • Large-scale SEC (MBG) – through collaboration with Jan Trige Rasmussen

EV Characterization

Nanoparticle tracking analysis (NTA)

  • Malvern NanoSight LM10-HS (iNANO)
  • Malvern NanoSight NS300 (AUH)

Dynamic light scattering (DLS)

  • Malvern ZetaSizer Nano (iNANO)

Flow cytometry (FCM) and cell sorter (FACS)

  • Sony ID7000 Spectral Cell Analyzer (FACS Core Facility, Biomed) – paid access or service
  • Amnis ImageStreamX MkII Imaging Flow Cytometer (FACS Core Facility, Biomed) – paid access or service
  • NovoCyte Quanteon (FACS Core Facility, Biomed) – paid access or service
  • Invitrogen Bigfoot Spectral Cell Sorter (FACS Core Facility, Biomed) – paid access or service

EV Imaging

Super-resolution microscopy

  • Oxford NanoImaging (ONI) microscope (iNANO) – through collaboration with Jørgen Kjems

Cryo-electron microscopy (Cryo-EM) and Cryo-correlative light-electron microscopy (Cryo-CLEM)

  • Titan Krios G3i (Embion) – paid access or service
  • Titan Krios (Embion) – paid access or service
  • Leica THUNDER Imager Cryo-CLEM (Embion) – paid access or service

EV Cargo Analysis

High-throughput analysis

  • RNAseq and bioinformatics (Omiics ApS) – paid service

Model Organisms

Non-mammalian

  • Zebrafish (MBG) – through collaboration with Yuya Hayashi

Associated Research Groups

A Zebrafish Approach to Extracellular Vesicle Biology

Key words: Bioimaging, EVomics, EV Characterization, Tissue Regeneration, Macrophage & Endothelial Biology

  • Department of Molecular Biology and Genetics

  • iNANO Interdisciplinary Nanoscience Center


EV Expertise

miRNA

Laboratoy Location

Universitetsbyen 81

Research Area

We study EVs as the new frontier of cell-to-cell communication. For example, by which mechanisms can EVs find the target recipient cells via the bloodstream? What kind of messages are conveyed to regulate/support the recipient cells? With zebrafish as our little partners, we seek answers to these questions by nanoscience approaches, bioinformatics and 4D imaging of live transgenic embryos.


Zebrafish models

We have two approaches: fluorescent labelling of endogenous EVs and microinjection of (fluorescently labelled) EVs including, but not limited to, human-derived EVs. In both cases, EVs are imaged in live zebrafish embryos using optical microscopes to study the biodistribution, blood clearance kinetics, and interactions with cells of interest (e.g. macrophages and endothelial cells). Induction of TNF-alpha can also be visualized in real-time as an indicator of macrophage polarization in response to EVs. Collaborations on tissue injury models and EV injections are welcome.

Immunoprecipitation of EVs

ChromoTek’s GFP-trap Dynabeads for EV isolation from tissues (zebrafish embryos).

Electron microscopy of EVs

Through Embion. Cryo-electron microscopy (cryo-EM) and cryo-correlative light-electron microscopy (cryo-CLEM) for imaging EVs at ultrastructural resolution.

XXXX

Key words: XXXX

  • Department of Molecular Biology and Genetics


EV Expertise

miRNA, Protein

Laboratoy Location

Universitetsbyen 81

Research Area

XXXX


Home-made size-exclusion chromatography

With a possibility for large-scale EV isolation.

Specific EV labelling

By lactadherin-fluorophore conjugates (binds to phosphatidylserine in the phospholipid membrane).

XXXX

Key words: XXXX

  • FACS Core Facility, Department of Biomedicine


EV Expertise

EV characterization

Laboratoy Location

Høegh-Guldbergs Gade 10

Research Area

XXXX


Sony ID7000 Spectral Cell Analyzer

Capable of resolving particles down to 160 nm on scatter. Johann Mar Gudbergsson has experience in analysing EVs with this.

Amnis ImageStreamX MkII Imaging Flow Cytometer

Capable of resolving particles down to 100 nm only by scatter but smaller when using fluorescence. Yuya Hayashi has experience in analysing EVs with this.

NovoCyte Quanteon

Capable of resolving particles down to 100 nm only by scatter but smaller when using fluorescence.

Invitrogen Bigfoot Spectral Cell Sorter

A cell sorter which has small particle detection mode enabling it to resolve/sort particles down to 100-160 nm – but the instrument is very new and we did not have time to test it yet. In theory, it should be possible to sort EVs on this.

XXXX

Key words: XXXX

  • iNANO Interdisciplinary Nanoscience Center

  • Department of Molecular Biology and Genetics

  • DANDRITE


EV Expertise

miRNA

Laboratoy Location

Gustav Wieds Vej 14

Research Area

XXXX


ONI microscopy

Nanoimager fluorescent and super-resolution microscope. Can image in both epifluorescence, total internal reflection (TIRF) and HILO. The microscope has four laser lines 405 nm, 488 nm, 561 nm, and 640 nm. The microscope can image in real-time ideal for tracking of single molecules and is equipped with a temperature controller and a micro fluiding pumping system. Read more here (https://oni.bio/nanoimager/).

Contact person for ONI: Mette Malle <malle@inano.au.dk>
OBS: the microscope is not trivial to use and Mette is happy to collaborate and/or help with everything from guiding which fluorophores should be used, microscope surface preparation and imaging conditions. 

Malvern NanoSight LM10-HS

Nanoparticle tracking analysis (NTA) for size and concentration determination (single-particle approach).

Malvern ZetaSizer Nano

Dynamic light scattering (DLS) for determination of size distributions (ensemble approach, intensity biased).

XXXX

Key words: XXXX

  • Department of Clinical Medicine


EV Expertise

miRNA, Protein

Laboratoy Location

Palle Juul-Jensens Boulevard 45

Research Area

XXXX


EV labelling

In vivo (pre-)labelling of EVs, engineered labelling of EVs, and engineered loading of therapeutic proteins into EVs

The CONAN assay

Augmented COlorimetric NANoplasmonic (CONAN) method for determining purity and concentration of EVs using gold nanoparticles.

Cell lines and primary cells

RAW264.7, SIM-A9, THP-1, HEK293T, IPEC-J2, PBMCs, Monocytes, Adipose derived stem cells (ASCs)

XXXX

Key words: XXXX

  • Department of Clinical Medicine


EV Expertise

XXXX

Laboratoy Location

Palle Juul-Jensens Boulevard 45

Research Area

XXXX


Izon qEV size-exclusion chromatography

For routine EV isolation.

Malvern NanoSight NS300

Nanoparticle tracking analysis (NTA) for size and concentration determination (single-particle approach). Available at Blood & Biochemistry, AUH.

XXXX

Key words: XXXX

  • Omiics ApS


Yan Yan

EV Expertise

miRNA

Laboratoy Location

Gustav Wieds Vej 14

Research Area

XXXX


RNAseq

Omiics can offer RNA sequencing service to EV samples and also offer suggestions on EV samples preparation for RNA sequencing. We have established bioinformatics pipeline for analyzing the small RNAs (miRNAs, tRNA derived small RNAs and other small RNAs) and long-RNAs (mRNAs, circular RNAs and long non-coding RNAs) in EV samples. The analysis include: quantification of different types of RNAs, differential expression analysis, pathway analysis and so on.