Ulf Andersson Vang Ørom

Ulf Andersson Vang Ørom

Lektor Institut for Molekylærbiologi og Genetik - Genekspression og Genmedicin

M ulf.orom@mbg.au.dk
H 1131, 526
P +4522288766

CV, publikationer, aktiviteter

Projekter for studerende

Nyhedsartikler

Research

Non-coding RNA

The lab focuses on non-coding RNA in the nucleus of the cell. We are interested in how non-coding RNAs are processed and mediate its functions. Of particular interest is protein-RNA interactions.

We use molecular biology techniques, RNA sequencing and bioinformatics. We also develop state-of-the-art techniques to use for specific scientific questions within our core competences.

The main topics of the lab are processing and regulation of primary miRNA biogenesis and the role of long non-coding RNA in transcriptional regulation with emphasis on enhancers.

For some of our work on these topics see:

http://rnajournal.cshlp.org/content/23/6/892.long
https://www.nature.com/articles/ncomms11212
https://www.tandfonline.com/doi/full/10.4161/15384101.2014.977641

Peer-reviewed articles

Sortér efter: Dato | Forfatter | Titel

Conrad, T., Ntini, E., Lang, B., Cozzuto, L., Andersen, J. B., Marquardt, J. U., Ponomarenko, J., Tartaglia, G. G. & Ørom, U. A. V. (2020). Determination of primary microRNA processing in clinical samples by targeted pri-miR-sequencing. RNA, 26(11), 1726-1730. https://doi.org/10.1261/rna.076240.120

Ørom, U. A. V. (2020). Preface. Methods in Molecular Biology, 2161, v.

Ntini, E. & Vang Ørom, U. A. (2020). Targeting Polyadenylation for Retention of RNA at Chromatin. Methods in molecular biology (Clifton, N.J.), 2161, 51-58. https://doi.org/10.1007/978-1-0716-0680-3_5

Xiao, H., Shiu, P. K. T., Gabryleska, M., Conn, S. J., Dey, A., Chakrabarti, K., Regouc, M., Pichler, M., Vang Ørom, U. A., Santulli, G., Nishiwada, S., Goel, A., Nagarajan, V., Timmons, L., Alahari, S. K., Laprovitera, N., Ferracin, M., Hu, P. & Jin, H. (2020). The non-coding rna journal club: Highlights on recent papers-7. Non-coding RNA, 5(2), [40]. https://doi.org/10.3390/NCRNA5020040

Louloupi, A. & Ørom, U. A. V. (2018). Inhibiting pri-miRNA processing with target site blockers. I U. Ørum (red.), miRNA Biogenesis (s. 63-68). Springer. Methods in Molecular Biology Bind 1823 https://doi.org/10.1007/978-1-4939-8624-8_6

Schmidt, E., Dhaouadi, I., Gaziano, I., Oliverio, M., Klemm, P., Awazawa, M., Mitterer, G., Fernandez-Rebollo, E., Pradas-Juni, M., Wagner, W., Hammerschmidt, P., Loureiro, R., Kiefer, C., Hansmeier, N. R., Khani, S., Bergami, M., Heine, M., Ntini, E., Frommolt, P. ... Kornfeld, J. W. (2018). LincRNA H19 protects from dietary obesity by constraining expression of monoallelic genes in brown fat. Nature Communications, 9(1), [3622]. https://doi.org/10.1038/s41467-018-05933-8

Ntini, E., Louloupi, A., Liz, J., Muino, J. M., Marsico, A. & Ørom, U. A. V. (2018). Long ncRNA A-ROD activates its target gene DKK1 at its release from chromatin. Nature Communications, 9, [1636]. https://doi.org/10.1038/s41467-018-04100-3

Louloupi, A. & Ørom, U. A. V. (2018). Metabolic pulse-chase RNA labeling for pri-miRNA processing dynamics. I U. Ørum (red.), miRNA Biogenesis (s. 33-41). Springer. Methods in Molecular Biology Bind 1823 https://doi.org/10.1007/978-1-4939-8624-8_3

Louloupi, A., Ntini, E., Conrad, T. & Ørom, U. A. V. (2018). Transient N-6-Methyladenosine Transcriptome Sequencing Reveals a Regulatory Role of m6A in Splicing Efficiency. Cell Reports, 23(12), 3429-3437. https://doi.org/10.1016/j.celrep.2018.05.077

Selected publications

Louloupi A., Ntini E., Liz J., Orom U.A.
Microprocessor dynamics shows co- and post-transcriptional processing of pri-miRNAs, RNA, 2017

Conrad T., Albrecht A.-S., Costa V.R.M., Sauer S., Meierhofer D., Orom U.A.
Serial interactome capture of the human cell nucleus, Nature Com., 2016

Vucicevic D., Corradin O., Ntini E., Scacheri P.C., Orom U.A.
Long ncRNA expression associates with tissue-specific enhancers, Cell cycle, 2015

Musahl A.S., Huang X., Rusakiewicz S., Ntini E., Marsico A., Kroemer G., Kepp O., Orom U.A.
A long non-coding RNA links calreticulin-mediated immunogenic cell removal to RB1 transcription. Oncogene. 2014.

Conrad, T., Marsico, A., Gehre, M., Orom, U.A.
Microprocessor activity controls differential miRNA biogenesis in vivo. Cell Reports. 2014.

Orom, U.A., Shiekhattar R.
Long noncoding RNAs usher in a new era in the biology of enhancers. Cell. http://dx.doi.org/10.1016/j.cell.2013.08.028

Marsico A, Huska MR, Lasserre J, Hu H, Vucicevic D, Musahl A, Orom U.A., Vingron M. 2013.
PROmiRNA: a new miRNA promoter recognition method uncovers the complex regulation of intronic miRNAs. Genome Biology. doi:10.1186/gb-2013-14-8-r84

Orom, U.A., Derrien, T., Beringer, M., Gumireddy, K., Gardini, A., Bussotti, G., Lai, F., Zytnicki, M.,
Notredame, C., Huang, Q., Guigo, R., and Shiekhattar, R. 2010.
Long non-coding RNAs with enhancer-like function in human cells. Cell 143(1):46-58.

Revideret 07.01.2021 -

Lisbeth Heilesen

Ulf Andersson Vang Ørom

Ulf Andersson Vang Ørom

Research

Forskning i medierne

Peer-reviewed articles

Selected publications