The Cap-binding complex (CBC) plays a key role in gene expression. During his PhD studies, Simone Giacometti used a diversity of approaches to elucidate the role of CBC associated proteins in RNA biogenesis and metabolism. The central focus of Simone’s project has been the utilization of an iCLIP approach to identify the transcriptome-wide targets for several RNA-binding proteins.

In order to characterize yet unknown CBC-interacting proteins, Simone also performed a protein interaction screen leveraging affinity capture, in combination with a formaldehyde-based chemical cross-linking strategy.

The PhD degree was completed at the Department of Molecular Biology and Genetics, Science and Technology, Aarhus University, Denmark in collaboration with the Institute of Molecular Genetics of Montpellier (IGMM), Université Montpellier 2, France.

This résumé is prepared by the PhD student.

Time: Monday 11 April 2016 at 14.00
Place: Building 1531, room 219, Auditorium D4, Aarhus University, Ny Munkegade 116, 8000 Aarhus C
Title of dissertation: CBC bound proteins and RNA fate
Contact information: Simone Giacometti, e-mail: giacometti@mb.au.dk, tel.: +1 646-639-0919
Members of the assessment committee:
Associate professor Peter Brodersen, Department of Biology, Copenhagen University, Denmark 
Research director 2nd class Inserm Stéphan Vagner, Insitute Curie, Paris, France
Research Director Jean-Christophe Andrau, Institut de Génétique Moléculaire de Montpellier, CNRS and Montpellier University, France 
Associate professor Anni Hangaard Andersen (chair), Department of Molecular Biology and Genetics, Aarhus University, Denmark
Main supervisor:
Professor Torben Heick Jensen Department of Molecular Biology and Genetics, Science and Technology, Aarhus University, Denmark
Co-supervisor:
Dr. Edouard Bertrand, Institut de Génétique Moléculaire de Montpellier, CNRS and Montpellier University, France 
Language: The PhD dissertation will be defended in English.

The defence is public.
The dissertation is available for reading at the Graduate School of Science and Technology/GSST, Ny Munkegade 120, building 1520, room 128-134, 8000 Aarhus C.