Michal Domanski: An optimized toolbox for capture of protein complexes

During his studies, Michal has developed a set of tools and approaches for the characterization of endogenous human complexes related to RNA metabolism. These new tools have facilitated an in-depth characterization of the RNA exosome interactome, revealing a hitherto unknown association with the 5’cap structure of RNAs. Additionaly, Michal has shown that assays previously restricted to recombinant proteins, can now be applied to protein complexes captured from an endognous source. The new findings contribute to our understanding of how gene expression is regulated at the postranscriptional level.

The PhD degree was completed at the Centre for mRNP Biogenesis and Metabolism, Department of Molecular Biology and Genetics, Aarhus University.

Time: Wednesday 18 December 2013 at 14.00
Place: Lecture Theatre G1 (1532-116), Department of Mathematics, Aarhus University
Title of dissertation: Tools and approaches for the characterization of the endogenous human exosome and its cofactors
Contact information: Michal Domanski, e-mail: domanski@mb.au.dk
Members of the assessment committee:
Dr. Markus Landthaler, Berlin Institute for Medical Systems Biology at the Max-Delbruck Center for Molecular Medicine, Germany Professor Jesper Velgaard Olsen, Novo Nordisk Foundation Center for Protein Research, Proteomics Program, Faculty of Health and Medical Sciences, University of Copenhagen, Denmark
Associate Professor Charlotte Rohde Knudsen (chair), Department of Molecular Biology and Genetics, Aarhus University
Main supervisor:
Professor Torben Heick Jensen, Centre for mRNP Biogenesis and Metabolism, Department of Molecular Biology and Genetics, Aarhus University
Co-supervisor:
Dr. John LaCava, Laboratory of Cellular and Structural Biology, Rockefeller University, USA
Language: The PhD dissertation will be defended in English.

The defence is public.
The dissertation is available for reading at the Graduate School of Science and Technology/GSST, Ny Munkegade 120, building 1521, room 112, 8000 Aarhus C.