Final adjustment of urine concentration occurs in the renal collecting duct where it is regulated by the antidiuretic hormone arginine vasopressin (AVP). Rapidly following AVP stimulation, aquaporin-2 (AQP2) is translocated from the intracellular vesicles to the apical plasma membrane instantly increasing water reabsorption and subsequent urine concentration. Water exits via basolaterally localized AQP3 and AQP4.

During her PhD studies, Saw Marlar set up an experimental system to study the short-term AVP regulation of AQP3. Using quantum dot (QD) labeling and k-space image correlation spectroscopy (kICS) approach, she observed that AQP3 is indeed regulated via changes in lateral diffusion within 15-30 minutes following AVP stimulation. The new finding indicates the occurrence of basolateral adaptations, which might aid in facilitating the AVP mediated increased water efflux. In addition, she established another experimental system to measure the lateral diffusion of AQP3 and AQP4 both in the lateral and basal membranes by use of micropatterning and kICS analysis.

The PhD degree was completed at the Department of Molecular Biology and Genetics, Science and Technology, AarhusUniversity.

Time: Thursday 27 March 2014 at 13.00

Place: 1110-223 Auditorium, Bioinformatics Research Centre

Title of dissertation: Plasma Membrane Diffusion of Aquaporin-3 in Epithelial Cells

Contact information: Saw Marlar, smarlar@mb.au.dk

Members of the assessment committee:

Associate professor Soichiro Yamada, Biomedical Engineering, UC Davis, CA, USA

Associate professor Daniel Wüstner, Department of Biochemistry and Molecular Biology, University of Southern Denmark, Denmark

Associate professor Anni Hangaard Andersen, Department of Molecular Biology and Genetics, Aarhus University, Denmark (chair)

Main supervisor:

Prof. Jan J. Enghild, Department of Molecular Biology and Genetics, Aarhus University, Denmark?

Co-supervisor:

Associate Professor Lene N. Nejsum, Department of Molecular Biology and Genetics, Aarhus University, Denmark?

Language: The dissertation will be defended in English

The defence is public
The dissertation is available for reading at the Graduate School of Science and Technology/GSST, Ny Munkegade 120, building 1521, room 112, 8000 Aarhus C.