Zeiss Axio Observer 7, widefield fluorescence microscope (inverted setup) 1874-360

Light source HPX120 Metal Halide Lamp

Objectives

20x 0.3               (LD A-Plan)                                                               4.3   mm

40x0.75              (Ec Plan Neofluar)                                                      0.71 mm          

63x 1.4 oil           (Plan apochromat)                                                     0.19 mm

100x1.4 oil          (Plan apochromat)                                                     0.17 mm          

DIC Optics

Filter sets                                       Excitation         Beamsplitter                                Emission

Alexa 488 /eGFP                        BP 450-490       FT 510                                             BP 515-565

Alexa 568 /mRFP                       BP 572/25         FT 590                                             BP 629/62

DAPI /Hoechst                            BP 390/40         FT420                                              BP 450/40

Alexa 555/ Cy3                           BP 545/25         FT 570                                             BP 605/70

Alexa 647/ CY5                           640/30               FT 660                                             BP 690/50

DIC

Motorized stage

37 C humidified influx chamber (no CO2)

Camera             Axiocam 702 mono