Zeiss Axioplan 2, widefield fluorescence microscope (upright setup) 1872-557

Light source Zeiss HBO 100 (Mercury vaporshort-arc lamp)

Objectives

2.5x 0.075         (EC Plan-Neofluar)                                                                              WD 9,5 mm

5x 0.17              (EC Plan-Neofluar)                                                                              WD   18,5 mm

10x 0.3              (EC Plan-Neofluar)                                                                              WD   5,2 mm

20x 0.5              (EC Plan-Neofluar)                                                                              WD   2,0 mm

40x 0.75            (Plan-Neofluar)                                                                                    WD   0,5 mm  

63x 0.95Korr      (Plan-Neofluar)                                                                                    WD   0.12 mm

Filter sets                                       Excitation         Beamsplitter                                Emission          

00 Alexa 568 / mcherry               BP 530-585       FT600                                              LP 615               

10 Alexa 488 /GFP                     BP 450-490       FT 510                                             BP515-565

20 Alexa 555 / dTomato               BP 546/12         FT560                                              BP575-640

13 EGFP                                    BP 470/20         FT495                                              BP505-530

34 Alexa 405 / BFP                     BP 390/22         FT420                                              BP460/50

DIC

Camera             Axiocam