Wednesday 1 October 2025 at 13:15-14:00
Followed by PhD-session at 14:30-15:00
(Coffee and cake will be served between lecture and PhD-session)
NUCLEUS (1871-120)
Host: Rasmus Kock Flygaard

High-resolution in situ structures of mammalian respiratory supercomplexes

Mitochondria are essential for ATP production via oxidative phosphorylation, involving respiratory complexes within the inner membrane. Despite extensive in vitro studies, understanding their mechanisms in a physiological environment is challenging due to the loss of the native membranes during purification. Here, we directly image porcine mitochondria by developing a hybrid in situ cryo-electron microscopy technique, which enabled us to determine the structures of various respiratory supercomplexes in the native membrane environment at near-atomic resolution.