Anna Ma?olepszy:LORE1 insertional mutagenesis in Lotus japonicus as a powerful tool to identify the putative gene involved in establishment of legume-rhizobia symbiosis
PhD defence, Monday 17 February 2014. Anna Ma?olepszy
During her studies, Anna Ma?olepszy was establishing the high-throughput set-up for LORE1 insertional mutagenesis in model legume Lotus japonicus. LORE1 is an endogenous retrotransposon, which can be epigenetically derepressed during tissue culture in Lotus. Thanks to the 2D plant set-up, pooling strategy and Illumina sequencing method, all the insertions can be easily identified in each line.
Subsequently Anna Ma?olepszy initiated the forward screening of LORE1 population and showed that it can be successfully used to identify the new genes involved in establishment of legumes-rhizobia symbiosis. The new alleles of well-known nodulation mutants were confirmed as well as the new symbiotic mutant was identified and examined further. These studies showed the successful establishment of powerful tool for mutagenesis, which ease the process of identification of gene function in Lotus.
The PhD degree was completed at the Department of Molecular Biology and Genetics (Centre for Carbohydrate Recognition and Signalling (CARB)), Science and Technology, Aarhus University.
Time: Monday 17 February 2014 at 13.00
Place: Meeting Room 2, AU conference centre, Fredrik Nielsens Vej 2-4
Title of dissertation: Establishing and screening of a Lotus japonicus LORE1 mutagenized population Contact information: Anna Ma?olepszy, e-mail: ann@mb.au.dk
Members of the assessment committee:
Associate Professor Fred Rook, Department of Plant and Environmental Sciences, Copenhagen University, Denmark
Dr. Pascal Ratet, Institut des Sciences du Végétal, CNRS, Gif sur Yvette Cedex, France
Professor Claus Oxvig (chair), Department of Molecular Biology and Genetics, Aarhus University, Denmark
Main supervisor:
Professor Jens Stougaard
Co-supervisor:
Dr. Stig U. Andersen
Language: The PhD dissertation will be defended in English.
The defence is public.
The dissertation is available for reading at the Graduate School of Science and Technology/GSST, Ny Munkegade 120, building 1521, room 112, 8000 Aarhus C.