Image acquisition:

Microscope stand (name and brand) + type of microscope (upright or inverted)

Acquisition software (include version)

Objective make / type / magnification / numerical aperture.

Detectors / camera(s)

Laser lines / light source used for excitation.

Filter set used / or if confocal range of emission wavelength collected.

For confocal images: include pixel size, scanning speed and direction and pin hole diameter.

z-stack range and intervals

Type of immersion oil

Timelapse information total imaging time and imaging frequency.

Image analysis:

Report the program used, include version number. Provide information about the workflow with sufficient details to allow method replication. This should include contrast enhancement, background subtraction, thresholding method, criteria used for object identification etc. If new tools are generated, consider uploading to GitHub or similar to make them available to others.  

Show example pictures either alongside graphs or as supplementary information.

If in doubt about how to find these information’s you are always welcome to ask, and I will gladly proofread the microscopy section of the methods.