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Zeiss Axio Observer D1, widefield fluorescence microscope (inverted setup)

Zeiss Axio Observer D1, widefield fluorescence microscope (inverted setup) Science park

Light source, Colibri led lasers 365, 470, 555 and 625

Objectives

2.5x 0.075         (EC Plan-Neofluar)                                                                               WD 9,5 mm

5x 0.17               (EC Plan-Neofluar)                                                                              WD   18,5 mm

10x 0.3               (EC Plan-Neofluar)                                                                              WD   5,2 mm

20x 0.4 (Corr) (LD Plan-Neofluar)                                                                                   WD   8,4 mm

40x 0.75            (Plan-Neofluar)

DIC

Filter cubes                                   Excitation         Beamsplitter                                Emission          

62 DAPI/Hoecst                          BP 370/40         TFT 395 + 495+ 610                    TBP 425 + 527 + LP 615

     Alexa 488                              BP 474/28

                                                  BP585/35                                                                   

43 Alexa 555 /Cy3                      545/25               FT570                                              605/70

77 EGFP                                   TBP 483 +        

     Dsred/Cy3                             564 +

     Alexa 647/Cy5                       642                      TFT 506+ 582+ 659                     TBP 526 + 601+ 688

Camera             Axiocam