Zeiss Axio Observer D1, widefield fluorescence microscope (inverted setup) Science park

Light source, Colibri led lasers 365, 470, 555 and 625

Objectives

2.5x 0.075         (EC Plan-Neofluar)                                                                               WD 9,5 mm

5x 0.17               (EC Plan-Neofluar)                                                                              WD   18,5 mm

10x 0.3               (EC Plan-Neofluar)                                                                              WD   5,2 mm

20x 0.4 (Corr) (LD Plan-Neofluar)                                                                                   WD   8,4 mm

40x 0.75            (Plan-Neofluar)

DIC

Filter cubes                                   Excitation         Beamsplitter                                Emission          

62 DAPI/Hoecst                          BP 370/40         TFT 395 + 495+ 610                    TBP 425 + 527 + LP 615

     Alexa 488                              BP 474/28

                                                  BP585/35                                                                   

43 Alexa 555 /Cy3                      545/25               FT570                                              605/70

77 EGFP                                   TBP 483 +        

     Dsred/Cy3                             564 +

     Alexa 647/Cy5                       642                      TFT 506+ 582+ 659                     TBP 526 + 601+ 688

Camera             Axiocam