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Bacteria’s secret weapon against pesticides and antibiotics revealed

Bacteria exhibit extreme adaptability, which makes them capable of surviving in the most inhospitable conditions. New research results produced by Danish and British researchers now reveal the molecular details behind one of the secret weapons used by bacteria in their battle to survive under very nutrient-poor and even toxic conditions.

Figure A. Methyl phosphate. B. Methyl phosphonate. Phosphonate compounds are characterised by a direct link between carbon (C) and phosphorus (P), marked with red. C. The molecular structure of the C-P lyase complex (Figure: Ditlev E. Brodersen, Aarhus University)
The Danish research team behind the article in Nature; from left: Bjarne Jochimsen, Lan Bich Van, Morten Kjeldgaard, Paulina Seweryn, Bjarne Hove-Jensen and Ditlev E. Brodersen (Photo: Lisbeth Heilesen, Aarhus University)
The British research team: Lori A. Passmore and Christopher J. Russo (Photo: Martin Phelps, Medical Research Council, Cambridge)

All living things need phosphate to grow, which is why several hundred million tons of phosphate fertilisers are used every year in agriculture throughout the world. The nutrient content is so low in many parts of the world’s oceans that all growth comes to a halt, and bacteria have therefore developed advanced mechanisms to extract phosphate from other substances. These are known as phosphonate compounds, which are produced by many primitive organisms and account for the largest known stock of phosphorus in the marine environment (see figure). Many of these compounds are formed as toxins (antibiotics) as part of the ongoing battle for survival among marine organisms. Several million kilograms of glyphosate (Roundup®) are used as pesticide in agriculture every year, and the accumulation of residues of this phosphonate compound in groundwater has led to growing concern in recent years.

Bacteria capable of converting phosphonate compounds into phosphate to boost their growth have developed an arsenal of fourteen proteins for this purpose, approximately half of which are enzymes required for the chemical transformation of the substances. Five of these enzymes accumulate in the cells in a large complex called the C-P lyase complex, which can catalyse two of the total of five reactions required to use the phosphonate compound for growth.

An international team consisting of researchers from both the Department of Molecular Biology and Genetics, Aarhus University, and the Medical Research Council (MRC) in Cambridge, UK, have now determined the precise molecular structure of the C-P lyase complex, making it possible for the first time to understand how the secret weapon used by bacteria actually works. Using X-ray crystallography and electron microscopy, the researchers were able to achieve extremely detailed insight into the structure of four of the enzymes, as well as the location of the fifth enzyme in the complex.

The results have just been published in the highly esteemed scientific journal Nature and they are expected to revolutionise our understanding of the way bacteria can survive under harsh natural conditions, as well as their ability to break down certain antibiotics. In the long term, the results could potentially be used to develop techniques for removing pesticide residues from drinking water, to avoid bacterial resistance to antibiotics, and to understand how the greenhouse effect is caused, as a significant amount of methane emission is due to bacterial conversion of methyl phosphonate in the world’s oceans.

Link to the scientific article in Nature: Structural insights into the bacterial carbon–phosphorus lyase machinery, Paulina Seweryn, Lan Bich Van, Morten Kjeldgaard, Christopher J. Russo, Lori A. Passmore, Bjarne Hove-Jensen, Bjarne Jochimsen & Ditlev E. Brodersen

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Associate Professor Ditlev E. Brodersen

Department of Molecular Biology and Genetics
Aarhus University, Denmark
- +45 2166 9001