The lab focuses on non-coding RNA in the nucleus of the cell. We are interested in how non-coding RNAs are processed and mediate its functions. Of particular interest is protein-RNA interactions.
We use molecular biology techniques, RNA sequencing and bioinformatics. We also develop state-of-the-art techniques to use for specific scientific questions within our core competences.
The main topics of the lab are processing and regulation of primary miRNA biogenesis and the role of long non-coding RNA in transcriptional regulation with emphasis on enhancers.
For some of our work on these topics see:
Louloupi A., Ntini E., Liz J., Orom U.A.
Microprocessor dynamics shows co- and post-transcriptional processing of pri-miRNAs, RNA, 2017
Conrad T., Albrecht A.-S., Costa V.R.M., Sauer S., Meierhofer D., Orom U.A.
Serial interactome capture of the human cell nucleus, Nature Com., 2016
Vucicevic D., Corradin O., Ntini E., Scacheri P.C., Orom U.A.
Long ncRNA expression associates with tissue-specific enhancers, Cell cycle, 2015
Musahl A.S., Huang X., Rusakiewicz S., Ntini E., Marsico A., Kroemer G., Kepp O., Orom U.A.
A long non-coding RNA links calreticulin-mediated immunogenic cell removal to RB1 transcription. Oncogene. 2014.
Conrad, T., Marsico, A., Gehre, M., Orom, U.A.
Microprocessor activity controls differential miRNA biogenesis in vivo. Cell Reports. 2014.
Orom, U.A., Shiekhattar R.
Long noncoding RNAs usher in a new era in the biology of enhancers. Cell. http://dx.doi.org/10.1016/j.cell.2013.08.028
Marsico A, Huska MR, Lasserre J, Hu H, Vucicevic D, Musahl A, Orom U.A., Vingron M. 2013.
PROmiRNA: a new miRNA promoter recognition method uncovers the complex regulation of intronic miRNAs. Genome Biology. doi:10.1186/gb-2013-14-8-r84
Orom, U.A., Derrien, T., Beringer, M., Gumireddy, K., Gardini, A., Bussotti, G., Lai, F., Zytnicki, M.,
Notredame, C., Huang, Q., Guigo, R., and Shiekhattar, R. 2010.
Long non-coding RNAs with enhancer-like function in human cells. Cell 143(1):46-58.