The centre has implemented a number of advanced techniques within molecular biology and biochemistry for isolation, cloning and purification of selected protein- and nucleic acid molecules from both bacteria (prokaryotes) and higher organisms (eukaryotes), including yeast. The techniques include e.g. high-throughput cloning methods, automated protein purification, and robotics-controlled crystallization.
Core facilities available at CSB, and the people to contact:
Crystallisation robots Ditlev E. Brodersen
Scientific computing and graphics Jesper Lykkegaard Karlsen
Selected protein and nucleic acid complexes are purified to a high degree of purity and used for biochemical analysis and crystallisation. The centre maintains automated techniques for setting up crystallisation experiments as well as an x-ray generator with image plate detector for direct, crystallographic analysis. Frequent access to international synchrotron radiation facilities, including the danish beamline Astrid, Cassiopeia at MAX-LAB in Lund, Sweden is also available, as well as synchrotrons in Germany, France and United Kingdom.
Crystallographic data processing and construction of molecular models and structural analysis is also done in-house and the centre has a number of powerful graphics workstations for this purpose.
