Differential Scanning Fluorimetry (DSF) / Dynamic Light Scattering (DLS)

• Nanotemper Prometheus Panta
  The Prometheus Panta is a state-of-the-art instrument for nanoscale differential scanning fluorimetry (DSF) and dynamic light scattering (DLS). Thermal stability is assessed by monitoring the spectral shift of tryptophan during protein unfolding, providing a melting temperature. The Panta processes 48 samples in parallel, making it well-suited for moderate-throughput screening. Common applications include protein formulation under stable conditions, mutant comparison, and ligand screening. The DLS module also measures particle size and homogeneity at a static temperature or during thermal unfolding.

• Nanotemper Tycho NT.6
  The Tycho NT.6 is a rapid alternative to the Panta, designed for quick assessment of sample quality between purification or treatment steps via thermal unfolding. It can process six capillaries simultaneously with a heating rate of 30 °C/min, yielding results in just 3 minutes. The resulting transition curves provide relative comparisons across samples, though they do not represent the true melting point of the protein due to the fast temperature ramp.

Mass Photometry

• Refeyn MPTwo
  The Refeyn MPTwo mass photometry instrument measures particle sizes in solution at the single-molecule level, making it ideal for analyzing heterogeneous mixtures of complexes. With a lower detection limit of 30 kDa, it is suited for samples at concentrations around 10 nM.

Dynamic Light Scattering (DLS) / Static Light Scattering (SLS)

• Malvern Zetasizer µV
  The Zetasizer µV is a cuvette-based system tailored for protein analysis, using dynamic light scattering (DLS) to measure particle size and static light scattering (SLS) to determine molecular weight.

• Nanotemper Prometheus Panta
  The Panta includes a DLS module that measures particle size and homogeneity either at a static temperature or during thermal unfolding, requiring only approximately 10 µl of sample loaded into a glass microcapillary.

Liquid Scintillation Counter

• Perkin-Elmer Tri-Carb 4910 TR
  The liquid scintillation counter detects radioactivity from various isotopes commonly used in biochemical assays, including ^3H and ^32P. All work with radioactive isotopes must adhere to local safety regulations.

Multimode Plate Reader

• Molecular Devices SpectraMax i3
  The multimode plate reader supports a range of optical measurements for biochemical assays, including absorbance, luminescence, fluorescence, FRET, and fluorescence polarization. Its plate format makes it ideal for high-throughput experiments.
• Clariostar Plus
  This multimode plate reader enables diverse optical measurements for biochemical assays, such as absorbance, luminescence, fluorescence, FRET, and fluorescence polarization. In addition to the SpectraMax, the instrument offers long-term shaking capabilities, atmospheric control, and temperature regulation up to 65 °C, enabling more complex and physiologically relevant assay conditions.

Microvolume Spectrophotometer / Cuvette Spectrophotometer / Microtube Fluorimeter

• DeNovix FX+
  This versatile instrument offers 1 µL UV-Vis, cuvette UV-Vis, and microtube fluorescence (for e.g. Qubit) capabilities. It is available without booking and free of charge.

Sample Preparation Instruments
All sample preparation instruments are available for use without booking and free of charge.

Centrifuge

• Eppendorf Centrifuge 5420
  A tabletop centrifuge designed for microtubes (1.5 and 2 ml) with a maximum speed of 20,000 rcf.

Microvolume Spectrophotometer / Cuvette Spectrophotometer / Microtube Fluorimeter

• DeNovix FX+
  This fully specced instrument offers 1 µL UV-Vis (nanodropesque), cuvette UV-Vis, and microtube fluorescence capabilities. It supports measurements of protein and RNA/DNA concentrations, colorimetric assays, and fluorescence-based kits.