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What we have published

The highly interdisciplinary nature of our research drives close collaborations with experts across different fields. Among them, the mainstream publications listed below are particularly relevant for the group's scientific focus area.

The Mainstream

The Protein Corona of Nanoparticles Determines Their Fate in the Bloodstream

"Tracing the in Vivo Fate of Nanoparticles with a 'Non-Self' Biological Identity"
ACS Nano 14. 10666–10679. 2020. DOI

Mohammad-Beigi H, Scavenius C, Jensen PB, Kjaer-Sorensen K, Oxvig C, Boesen T, Enghild JJ, Sutherland DS, Hayashi Y*.

We show how non-self protein coronas can determine the blood clearance kinetics of the nanoparticles leading to vascular damages and inflammation.

Nanoparticle Uptake Visualized in Real-Time and at High-Resolution Using a Zebrafish Model

"Differential Nanoparticle Sequestration by Macrophages and Scavenger Endothelial Cells Visualized In Vivo in Real-Time and at Ultrastructural Resolution"
ACS Nano 14. 1665-1681. 2020DOI

Hayashi Y*, Takamiya M, Jensen PB, Ojea-Jiménez I, Claude H, Antony C, Kjaer-Sorensen K, Grabher C, Boesen T, Gilliland D, Oxvig C, Strähle U, Weiss C.

This article summarizes our imaging approaches to study the biological fate of nanoparticles with a particular emphasis on macrophage and endothelial biology.

Other Publications

Nanoparticle-protein corona

Mohammad-Beigi H, Hayashi Y, Moeslund Zeuthen C, Eskandari H, Scavenius C, Juul-Madsen K, Vorup-Jensen T, Enghild JJ, Sutherland DS*.
Mapping and identification of soft corona proteins at nanoparticles and their impact on cellular association.
Nature Communications 11. 4535. 2020. DOI

Hayashi Y*, Miclaus T, Murugadoss S, Takamiya M, Scavenius C, Kjaer-Sorensen K, Enghild JJ, Strähle U, Oxvig C, Weiss C, Sutherland DS*.
Female versus male biological identities of nanoparticles determine the interaction with immune cells in fish.
Environmental Science: Nano 4. 895-906. 2017. DOI

Zebrafish/vascular biology

Wild R, Klems A, Takamiya M, Hayashi Y, Strähle U, Ando K, Mochizuki N, Steib A, van Impel A, Schulte-Merker S, Krueger J, Préau L, le Noble F*.
Neuronal sFlt1 and Vegfaa determine venous sprouting and spinal cord vascularization.
Nature Communications 8. 13991. 2017. DOI

Earthworm immunity

Bodó K, Hayashi Y, Gerencsér G, László Z, Kéri A, Galbács G, Telek E, Mészáros M, Deli MA, Kokhanyuk B, Németh P, Engelmann P*.
Species-specific sensitivity of Eisenia earthworms towards noble metal nanoparticles: a multiparametric in vitro study. 
Environmental Science: Nano 7. 3509-3525. 2020. DOI

Bodó K, Baranzini N, Girardello R, Kokhanyuk B, Németh P, Hayashi Y, Grimaldi A, Engelmann P*.
Nanomaterials and Annelid Immunity: A Comparative Survey to Reveal the Common Stress and Defense Responses of Two Sentinel Species to Nanomaterials in the Environment.
Biology 9. 307. 2020. DOI

Engelmann P*, Hayashi Y, Bodó K, Ernszt D, Somogyi I, Steib A, Orban J, Pollák E, Nyitrai M, Németh P, Molnár L.
Phenotypic and functional characterization of earthworm coelomocyte subsets: Linking light scatter-based cell typing and imaging of the sorted populations.
Developmental & Comparative Immunology 65. 41-52. 2016. DOI

Yuya's Pre-PhD Publications

Hayashi Y, Miclaus T, Engelmann P, Autrup H, Sutherland DS, Scott-Fordsmand JJ*.
Nanosilver pathophysiology in earthworms: Transcriptional profiling of secretory proteins and the implication for the protein corona.
Nanotoxicology 10. 303-311. 2016DOI

Engelmann P*, Hayashi Y, Bodó K, Molnár L.
New Aspects of Earthworm Innate Immunity: Novel Molecules and Old Proteins With Unexpected Functions.
In ”Lessons in Immunity: From Single-Cell Organisms to Mammals”; Ballarin L, Cammarata M. Eds.; Academic Press 53-66. 2016.

Hayashi Y*, Miclaus T, Scavenius C, Kwiatkowska K, Sobota A, Engelmann P, Scott-Fordsmand JJ, Enghild JJ, Sutherland DS*.
Species Differences Take Shape at Nanoparticles: Protein Corona Made of the Native Repertoire Assists Cellular Interaction.
Environmental Science & Technology 47. 14367-14375. 2013. [featured on the journal cover]

Hayashi Y* and Engelmann P.
Earthworm’s immunity in the nanomaterial world: new room, future challenges.
Invertebrate Survival Journal 10. 69-76. 2013.

Hayashi Y, Heckmann LH, Simonsen V, Scott-Fordsmand JJ*.
Time-course profiling of molecular stress responses to silver nanoparticles in the earthworm Eisenia fetida.
Ecotoxicology and Environmental Safety 98. 219-226. 2013.

Jiang X, Foldbjerg R, Miclaus T, Wang L, Singh R, Hayashi Y, Sutherland DS, Chen C, Autrup H, Beer C*.
Multi-platform genotoxicity analysis of silver nanoparticles in the model cell line CHO-K1.
Toxicology Letters 222. 55-63. 2013.

Foldbjerg R, Irving ES, Hayashi Y, Sutherland DS, Thorsen K, Autrup H, Beer C*.
Global gene expression profiling of human lung epithelial cells after exposure to nanosilver.
Toxicological Sciences 130. 145-157. 2012.

Hayashi Y*, Engelmann P, Foldbjerg R, Szabó M, Somogyi I, Pollák E, Molnár L, Autrup H, Sutherland DS, Scott- Fordsmand JJ, Heckmann LH.
Earthworms and Humans in Vitro: Characterizing Evolutionarily Conserved Stress and Immune Responses to Silver Nanoparticles.
Environmental Science & Technology 46. 4166-4173. 2012.

Beer C*, Foldbjerg R, Hayashi Y, Sutherland DS, Autrup H.
Toxicity of silver nanoparticles – nanoparticle or silver ion?
Toxicology Letters 208. 286-292. 2012.

Hayashi Y, Heckmann LH*, Callaghan A, Sibly RM.
Reproduction recovery of the crustacean Daphnia magna after chronic exposure to ibuprofen.
Ecotoxicology 17. 246-251. 2008.