Institut for Molekylærbiologi og Genetik

Aarhus Universitets segl

Jan J. Enghild

Research project: Proteome analysis and protein characterization

The laboratory seeks to describe the "structure–activity–function" algorithm of proteins. Our research is located at the interface between other structure analyses methods (NMR and X-ray crystallography) and we use a combination of protein chemistry, proteomics, enzymology, and recombinant DNA techniques.


We are interested in extracellular matrix (ECM) homeostasis and the identification and characterization of post-translational modifications. Available structural and functional information concerning ECM proteins is limited due to the inherent insolubility.

Granular corneal dystrophy, Type I. 

A superposition of the crystal structures of bovine TAFI (green) and porcine carboxypeptidase B (purple). Read more.

Protein Characterization

Our research is located at the interface between other structure analyses methods (NMR and X-ray crystallography) and we use a combination of protein chemistry, proteomics, enzymology, and recombinant DNA techniques.

  • Bikunin Proteins
  • Extracellular superoxide dismutase (EC-SOD)
  • Matrix metalloproteinase (MMP)
  • Novel proteolytic enzymes
  • Pigment epithelium-derived factor (PEDF)
  • Thrombin Activatable Fibrinolysis inhibitor (TAFI)
  • Transforming growth factor beta induced protein (TGFBIP) and cornea dystrophies
  • Transglutaminase

Peer-reviewed articles

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Fattman, C. L., Chu, C. T., Kulich, S. M., Enghild, J. J. & Oury, T. D. (2001). Altered expression of extracellular superoxide dismutase in mouse lung after bleomycin treatment. Free Radic. Biol. Med., 31, 1198-1207.
Fattman, C. L., Enghild, J. J., Crapo, J. D., Schaefer, L. M., Valnickova, Z. & Oury, T. D. (2000). Purification and immunolocalization of extracellular superoxide dismutase in mouse lung. Biochem. Biophys. Res. Commun., 274, 542-548.
Fattman, C. L., Enghild, J. J., Crapo, J. D., Schaefer, L. M., Valnickova, Z. & Oury, T. D. (2000). Purification and characterization of extracellular superoxide dismutase in mouse lung. Biochemical and Biophysical Research Communications, 275(2), 542-548. https://doi.org/10.1006/bbrc.2000.3327
Falkowski, K., Bielecka, E., Thøgersen, I. B., Bocheńska, O., Płaza, K., Kalińska, M., Sąsiadek, L., Magoch, M., Pęcak, A., Wiśniewska, M., Gruba, N., Wysocka, M., Wojtysiak, A., Brzezińska-Bodal, M., Sychowska, K., Pejkovska, A., Rehders, M., Butler, G., Overall, C. M. ... Kantyka, T. (2020). Kallikrein-Related Peptidase 14 Activates Zymogens of Membrane Type Matrix Metalloproteinases (MT-MMPs)-A CleavEx Based Analysis. International Journal of Molecular Sciences , 21(12), 1-22. Artikel 4383. https://doi.org/10.3390/ijms21124383
Falkenberg, C., Enghild, J. J., Thøgersen, I. B., Salvesen, G. & Akerström, B. (1994). Isolation and characterization of fibronectin-alpha 1-microglobulin complex in rat plasma. Biochemical Journal, 301 ( Pt 3), 745-51.
Falkenberg, C., Allhorn, M., Thøgersen, I. B., Valnickova, Z., Pizzo, S. V., Salvesen, G., Akerström, B. & Enghild, J. J. (1995). alpha 1-Microglobulin destroys the proteinase inhibitory activity of alpha 1-inhibitor-3 by complex formation. Journal of Biological Chemistry, 270(9), 4478-83.
Erxleben, D. A., Dodd, R. J., Day, A. J., Green, D. E., DeAngelis, P. L., Poddar, S., Enghild, J. J., Huebner, J. L., Kraus, V. B., Watkins, A. R., Reesink, H. L., Rahbar, E. & Hall, A. R. (2024). Targeted Analysis of the Size Distribution of Heavy Chain-Modified Hyaluronan with Solid-State Nanopores. Analytical Chemistry, 96(4), 1606-1613. https://doi.org/10.1021/acs.analchem.3c04387
Englert, J. M., Ramsgaard, L., Valnickova, Z., Enghild, J. J. & Oury, T. D. (2008). Large scale isolation and purification of soluble RAGE from lung tissue. Protein Expression and Purification, 61(1), 99-101. https://doi.org/10.1016/j.pep.2008.05.004
Enghild, J. J., Berkowitz, P., Hu, P., Liu, L., Diaz, L. A., Chua, M. P. & Rubenstein, D. S. (2005). Desmosome signaling. Inhibition of p38MAPK prevents pemphigus vulgaris IgG-induced cytoskeleton reorganization. Journal of Biological Chemistry, 280, 23778-23784.
Enghild, J. J., Berggard, T., Miron, S., Onnerfjord, P., Thulin, E., Akerfeldt, KS., Enghild, J. J., Akke, M. & Linse, S. (2002). Calbindin D28k exhibits properties characteristic of a Ca2+ sensor. Journal of Biological Chemistry, 277(19), 16662-72.

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Revideret 15.08.2023
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Lisbeth Heilesen